Radiotherapy is a standard methodology to deal with gastric most cancers (GC). Nevertheless, the medical outcomes of GC radiotherapy face challenges, and the mechanisms of GC radioresistance stay unclear.
Our research aimed to analyze the function and mechanism of miR-4537 within the radiation sensitivity of GC cells. Cell viability was decided by Cell Counting Equipment-8. The proliferation of HGC27 and KATO III cells was measured utilizing a colony formation assay.
Movement cytometry was carried out to look at the adjustments in cell apoptosis. Western blotting was carried out to detect the expression of zinc finger protein 587 (ZNF587) protein in HGC27 and KATO III cells.
To verify the connection between miR-4537 and ZNF587, a luciferase reporter assay was carried out. MiR-4537 was downregulated in GC tumors and cells and suppressed cell proliferation, whereas selling cell apoptosis in GC. Importantly, we discovered that miR-4537 lowered the radioresistance of GC cells.
As well as, we additionally confirmed that miR-4537 expression is negatively correlated with ZNF587 expression in GC tissues. MiR-4537 sure to ZNF587 and suppressed the expression degree of ZNF587.
Overexpression of ZNF587 partially counteracted the consequences of miR-4537 on cell proliferation and apoptosis. In conclusion, in GC cells, miR-4537 inhibited the power of cell proliferation, however quite the opposite, it promoted the power of cell apoptosis and improved radiosensitivity of the cells.
| Custom Antibody titration by ELISA up to 2 rabbits and 1 bleed | |||
| ELISA-1 | |||
| Beta2-Microglobulin ELISA kit ELISA Kit | |||
| LF-EK60047 | |||
| Chicken thrombomodulin,TM ELISA KIT ELISA | |||
| QY-E80092 | |||
| Oxycodone ELISA | |||
| EK7130 | |||
| Amphiphysin ELISA | |||
| LF-EK0189 | |||
MicroRNA-382-5p inhibits osteosarcoma growth and development by negatively regulating VEZF1 expression
Human osteosarcoma is essentially the most frequent malignant main bone tumor that primarily happens in younger adults and youngsters. MicroRNAs (miRNAs/miRs) are abnormally expressed in human osteosarcoma and contribute to osteosarcoma initiation and growth.
The current research aimed to analyze the function of miR-382-5p within the nosogenesis of osteosarcoma and to establish a novel goal for osteosarcoma remedy. miR-382-5p expression was detected in human osteosarcoma medical tissues and cell strains, together with 143B, U2OS and MG63, by way of reverse transcription-quantitative PCR evaluation.
A number of bioinformatic prediction toowe used to establish the potential goal genes of miR-382-5p and vascular endothelial zinc finger 1 (VEZF1), which have been validated by way of the dual-luciferase reporter assay. MG63 and U2OS cells have been transfected with miR-382-5p mimics.
The Cell Counting Equipment-8 assay was carried out to evaluate cell proliferation, whereas the Transwell assay was carried out to evaluate migration and invasion. Cell colony formation was measured by way of crystal violet staining, and apoptosis was assessed by way of Annexin V/propidium iodide staining.
The wound therapeutic assay was carried out to evaluate the migratory skill of U2OS and MG63 cells. Antitumor results of miR-382-5p have been evaluated in nude mice xenografts utilizing U2OS cells. The outcomes demonstrated that miR-382-5p expression was markedly downregulated in human osteosarcoma tissues and cell strains in contrast with adjoining regular tissues.
Transfection of miR-382-5p mimics into MG63 and U2OS cells considerably inhibited the malignant behaviors of cells, together with decreased proliferation, migration, diminished colony formation and invasion, and promoted osteosarcoma cell apoptosis.
Bioinformatics prediction indicated that VEZF1 is a direct goal gene of miR-382-5p. Overexpression of VEZF1 restored osteosarcoma tumor growth inhibited by miR-382-5p in vivo.
As well as, overexpression of miR-382-5p restrained the expansion of xenograft osteosarcoma in nude mice following co-transfection, and overexpression of VEZF1 attenuated the inhibitory impact of miR-382-5p in nude mice.
miR-382-5p acted as a tumor suppressor gene and inhibited the malignant organic behaviors of human osteosarcoma cells and features related to straight concentrating on VEZF1. Taken collectively, these outcomes counsel that the miR-382-5p/VEZF1 interplay has an vital function in osteosarcoma growth and development, and thus could also be used as a diagnostic and therapeutic goal for osteosarcoma.
lncRNA ZFAS1 promotes the ox-LDL induced proliferation, invasion and migration of vascular smooth muscle cells
Atherosclerosis is a chronic progressive inflammatory vascular disease. The dysfunction of vascular smooth muscle cells (VSMCs) induced by oxidized low-density lipoprotein (ox-LDL) contributes to the formation of atherosclerotic lesions.
Additionally, upregulation of the long non-coding RNA zinc finger antisense 1 (ZFAS1) was observed in the plaques of patients with atherosclerosis. The aim of the present study was to explore the functional role of ZFAS1 in atherosclerosis progression.
Reverse transcription-quantitative PCR was performed to analyze ZFAS1 mRNA expression, and western blotting was performed to determine the protein expression levels of Ki67, proliferating cell nuclear antigen (PCNA), matrix metallopeptidase (MMP)2 and MMP9.
The Cell Counting Kit-8 assay was used to test cell viability. Finally, wound healing and Transwell chamber assays were performed to evaluate cell migration and invasion, respectively. The current findings demonstrated that ZFAS1 expression was upregulated by ox-LDL stimulation in VSMCs.
Moreover, ZFAS1 overexpression promoted the ox-LDL-induced proliferation, migration and invasion of VSMCs, and upregulated the expression levels of proteins associated with cellular proliferation (Ki67 and PCNA), migration and invasion (MMP2 and 9).
By contrast, ZFAS1-knockdown inhibited the proliferation, migration and invasion of VSMCs, and suppressed cell proliferation-, migration- and invasion-associated protein expression. In conclusion, ZFAS1 promoted the ox-LDL-induced proliferation, invasion and migration of VSMCs. Thus, ZFAS1 may represent a novel biomarker for dysfunction of VSMCs in the pathological condition of atherosclerosis.
Long non-coding RNA ZFAS1 alleviates bupivacaine-induced neurotoxicity by regulating the miR-421/zinc finger protein564 (ZNF564) axis
This research aimed to explore the biological role of long non-coding RNA (lncRNA) ZFAS1 in bupivacaine-induced neurotoxicity. The levels of lncRNA ZFAS1, miR-421, and zinc finger protein 564 (ZNF564) were detected by RT-qPCR.
MTT and TUNEL assays were utilized to evaluate cell viability and apoptosis, respectively. Caspase-3 activity was measured by the caspase-3 activity assay kit. The binding ability between miR-421 and ZFAS1 or ZNF564 was confirmed by Rip and dual-luciferase reporter assays.
In this study, it was found that the levels of ZFAS1 and ZNF564 were gradually upregulated and miR-421 expression was downregulated with increasing concentrations of bupivacaine. Functional assays indicated that the silencing of ZFAS1 suppressed cell viability and facilitated cell apoptosis of SH-SY5Y cells, while overexpression of ZFAS1 had the opposite effects.
Moreover, it was identified that miR-421 was a target of ZFAS1, and ZFAS1 regulated the bupivacaine-induced neurotoxicity via miR-421. In addition, we confirmed that ZNF564 was a downstream target of miR-421.
The upregulation of miR-421 decreased the cell viability, and increased the cell apoptosis rate and caspase-3 activity, while the upregulation of ZND564 partially abolished these effects.
Finally, it was demonstrated that ZFAS1 could upregulate the expression of ZNF564 by targeting miR-421. In conclusion, our results demonstrated that ZFAS1 alleviated bupivacaine-induced neurotoxicity through the miR-421/ZNF564 axis, suggesting a new strategy for the amelioration of bupivacaine-induced neurotoxicity.
TAGLN2 promotes the proliferation, invasion, migration and epithelial-mesenchymal transition of colorectal most cancers cells by activating STAT3 signaling via ANXA2
Colorectal most cancers (CRC) is among the main causes of cancer-associated mortality worldwide and at the moment ranks third within the USA by way of prevalence. Transgelin-2 (TAGLN2) was beforehand reported to function a tumor promoter in numerous varieties of most cancers.
The current research aimed to analyze the function of TAGLN2 within the development of CRC and to find out the potential underlying mechanism. The expression degree of TAGLN2 in CRC cells (HCT116, SNU-C1, LoVo and SW480) have been first detected by reverse transcription quantitative PCR and western blotting.
Following TAGLN2 knockdown via transfection with brief hairpin (sh)RNAs towards TAGLN2, CRC cell proliferation was decided utilizing Cell Counting Equipment-Eight and 5′-ethynyl-2′-deoxyuridine assays. Cell migration and invasion have been evaluated utilizing wound therapeutic and Transwell assays, respectively.
The expression ranges of matrix metalloproteinase (MMP)2, MMP9 and proteins related to epithelial-mesenchymal transition (EMT), together with N-cadherin (N-cad), vimentin, zinc finger E-box binding homeobox 2 (ZEB2) and E-cadherin (E-cad), have been additionally evaluated by western blotting.
Moreover, following TAGLN2 overexpression and using sign transducer and activator of transcription 3 (STAT3) inhibitors to deal with CRC cells, all of the aforementioned organic parameters have been evaluated.
The potential relationship between annexin 2 (ANXA2) and STAT3 was confirmed by western blotting evaluation. The expression degree of TAGLN2 was discovered to be significantly excessive in CRC cells. Following TAGLN2 knockdown, CRC cell proliferation, migration, invasion and EMT have been considerably inhibited.
TAGLN2 knockdown additionally suppressed STAT3 phosphorylation in CRC cells. As well as, the selling results of TAGLN2 overexpression on the development of CRC have been reversed by STAT3 inhibitor. Moreover, ANXA2 was positively related to STAT3.
Taken collectively, these findings demonstrated that TAGLN2 might promote the proliferation, invasion, migration and EMT of CRC cells by activating STAT3 and regulating ANXA2 expression.
DNA Assay Kit |
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| 6023 | Chondrex | 1 kit | EUR 216.6 |
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Description: DNA Assay Kit |
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HAT Assay Kit |
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| 55R-1373 | Fitzgerald | 100 assays | EUR 888 |
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Description: Assay Kit for detection of HAT in the research laboratory |
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SOD Assay Kit |
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| 55R-1374 | Fitzgerald | 100 assays | EUR 722.4 |
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Description: Assay Kit for detection of SOD in the research laboratory |
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ATP Assay Kit |
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| 55R-1380 | Fitzgerald | 100 assays | EUR 970.8 |
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Description: Assay Kit for detection of ATP in the research laboratory |
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ADP Assay Kit |
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| 55R-1381 | Fitzgerald | 100 assays | EUR 970.8 |
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Description: Assay Kit for detection of ADP in the research laboratory |
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FAD Assay Kit |
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| 55R-1382 | Fitzgerald | 100 assays | EUR 878.4 |
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Description: Assay Kit for detection of FAD in the research laboratory |
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PEP Assay Kit |
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| 55R-1384 | Fitzgerald | 100 assays | EUR 1051.2 |
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Description: Assay Kit for detection of PEP in the research laboratory |
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JNK Assay Kit |
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| 55R-1407 | Fitzgerald | 40 assays | EUR 1041.6 |
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Description: Assay Kit for detection of JNK in the research laboratory |
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JNK Assay Kit |
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| 55R-1408 | Fitzgerald | 40 assays | EUR 1174.8 |
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Description: Assay Kit for detection of JNK in the research laboratory |
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Akt Assay Kit |
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| 55R-1409 | Fitzgerald | 40 assays | EUR 1174.8 |
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Description: Assay Kit for detection of Akt in the research laboratory |
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MDA Assay Kit |
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| 55R-1512 | Fitzgerald | 100 assays | EUR 970.8 |
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Description: Assay Kit for detection of MDA in the research laboratory |
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PAH Assay Kit |
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| 55R-1547 | Fitzgerald | 100 assays | EUR 888 |
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Description: Assay Kit for detection of PAH in the research laboratory |
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POP Assay Kit |
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| 80106 | BPS Bioscience | 96 rxns. | EUR 685 |
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Description: The Fluorogenic Prolyl OligoPeptidase (POP)_x000D_ Assay Kit is a complete assay system designed to measure activity of the purified POP_x000D_ enzyme. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive_x000D_ materials and chromatography in traditional assays. Purified human recombinant POP is_x000D_ included in the kit as a positive control. Using this kit, only one simple step, in which the_x000D_ fluorometric substrate is incubated with purified POP, is needed to analyze the POP_x000D_activity level. The resulting fluorescent product can then be easily measured with a_x000D_ microtiter-plate fluorimeter. |
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FAP Assay Kit |
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| 80210 | BPS Bioscience | 96 rxns. | EUR 1175 |
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Description: The Fluorogenic FAP Assay Kit is designed to measure FAP activity using purified_x000D_FAP for screening and profiling applications. _x000D_The key to the Fluorogenic FAP Assay Kit is the fluorogenic substrate. Using this kit,_x000D_only one simple step on a microtiter plate is required for FAP reactions. The DPP_x000D_fluorometric substrate is incubated with a sample containing FAP enzyme to produce a_x000D_fluorophore that can then be measured using a fluorescence reader. |
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FYN Assay Kit |
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| 78003 | BPS Bioscience | 96 rxns. | EUR 535 |
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Description: The FYN Assay Kit is designed to measure FYN activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. |
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RET Assay Kit |
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| 79566 | BPS Bioscience | 96 rxns. | EUR 535 |
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Description: RET is a receptor kinase for GDNF (glial cell-line derived neurotrophic factor) family ligands (GFLs). Activation of wild-type RET is important in several cancers where it contributes to tumor progression through various mechanisms. In addition, it has been shown that activating mutation as well as RET rearrangement that leads constitutively active protein plays a significant role in various cancer types. Importantly, small molecule inhibitors of RET have been clinically proved as a promising therapeutic reagent in medullary thyroid cancer and are being evaluated for other types of cancers related to RET overexpression or mutation. The RET Assay Kit is designed to measure RET activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The RET Assay Kit comes in a convenient 96-well format, with enough purified recombinant RET enzyme, RET substrate peptide (IRF-1Rtide), ATP and kinase assay buffer for 100 enzyme reactions. |
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BTK Assay Kit |
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| 79568 | BPS Bioscience | 96 rxns. | EUR 560 |
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Description: Bruton's tyrosine kinase or BTK, is an enzyme that plays a role in the functionality and maturation of B cells. The BTK pathway has implications for a number of autoimmune disorders including isolated growth hormone deficiency type III and rheumatoid arthritis. The BTK Assay Kit is designed to measure BTK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The BTK Assay Kit comes in a convenient 96-well format, with enough purified recombinant BTK enzyme, PolyGluTyr peptide, ATP, and kinase assay buffer for 100 enzyme reactions. |
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SYK Assay Kit |
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| 79671 | BPS Bioscience | 96 rxns. | EUR 525 |
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Description: SYK of the nonreceptor tyrosine kinases plays a role in autoimmune diseases like Epstein Barr virus and hematopoietic malignancies. The SYK Assay Kit is designed to measure SYK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. |
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SRC Assay Kit |
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| 79680 | BPS Bioscience | 96 rxns. | EUR 535 |
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Description: SRC is a member of the nonreceptor tyrosine kinases that plays a role in many cellular functions, including cell adhesion, growth, and differentiation. SRC has been implicated in diseases such as chronic kidney disease and metastatic bone disease. The SRC Assay Kit is designed to measure SRC activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. The SRC Assay Kit comes in a convenient 96-well format, with enough purified recombinant SRC enzyme, Protein Tyrosine Kinase Substrate (Poly-Glu,Tyr 4:1), ATP, and kinase assay buffer for 100 enzyme reactions. |
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LCK Assay Kit |
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| 79794 | BPS Bioscience | 96 rxns. | EUR 535 |
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Description: The LCK Assay Kit is designed to measure LCK activity for screening and profiling applications using Kinase-Glo® MAX as a detection reagent. |
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ADA Assay Kit |
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| abx098403-Hitachi7060R190ml2R290ml1 | Abbexa | Hitachi 7060; R1: 90ml×2 R2: 90ml×1 | EUR 886.8 |
ADA Assay Kit |
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| abx098403-Hitachi7170R140ml4R220ml4 | Abbexa | Hitachi 7170; R1: 40ml×4 R2: 20ml×4 | EUR 961.2 |
ADA Assay Kit |
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| abx098403-Hitachi7170R160ml4R260ml2 | Abbexa | Hitachi 7170; R1: 60ml×4 R2: 60ml×2 | EUR 1093.2 |
ADA Assay Kit |
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| abx098403-Toshiba40R150ml4R250ml2 | Abbexa | Toshiba 40; R1: 50ml×4 R2: 50ml×2 | EUR 943.2 |
ADA Assay Kit |
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| abx090675-100tests | Abbexa | 100 tests | EUR 284.4 |
DNA Assay Kit |
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| Z5030023 | Biochain | 250 assays | EUR 636 |
ATP Assay Kit |
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| Z5030041 | Biochain | 100 assays | EUR 636 |
HDAC Assay Kit |
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| 55R-1371 | Fitzgerald | 100 assays | EUR 807.6 |
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Description: Assay Kit for detection of HDAC in the research laboratory |
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HDAC Assay Kit |
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| 55R-1372 | Fitzgerald | 100 assays | EUR 807.6 |
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Description: Assay Kit for detection of HDAC in the research laboratory |
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cAMP Assay Kit |
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| 55R-1389 | Fitzgerald | 100 assays | EUR 847.2 |
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Description: Assay Kit for detection of cAMP activity in the research laboratory |
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cGMP Assay Kit |
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| 55R-1390 | Fitzgerald | 100 assays | EUR 847.2 |
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Description: Assay Kit for detection of cGMP activity in the research laboratory |
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Urea Assay Kit |
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| 55R-1391 | Fitzgerald | 100 assays | EUR 888 |
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Description: Assay Kit for detection of Urea in the research laboratory |
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Iron Assay Kit |
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| 55R-1395 | Fitzgerald | 100 assays | EUR 909.6 |
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Description: Assay Kit for detection of Iron in the research laboratory |
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Heme Assay Kit |
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| 55R-1492 | Fitzgerald | 100 assays | EUR 807.6 |
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Description: Assay Kit for detection of Heme in the research laboratory |
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DPP4 Assay Kit |
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| 55R-1526 | Fitzgerald | 100 assays | EUR 900 |
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Description: Assay Kit for detection of DPP4 in the research laboratory |
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MMP3 Assay Kit |
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| 55R-1530 | Fitzgerald | 100 assays | EUR 867.6 |
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Description: Assay Kit for detection of MMP3 in the research laboratory |
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DPP3 Assay Kit |
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| 80203 | BPS Bioscience | 96 rxns. | EUR 615 |
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Description: The Fluorogenic DPP3 Assay Kit is designed to measure DPP3 activity using purified DPP3 for screening or profiling applications. The key to the Fluorogenic DPP3 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP3 reactions; no time-consuming washing steps are required. The fluorometric substrate is incubated with a sample containing DPP3 enzyme to produce a fluorophore that can then be measured using a fluorescence reader. |
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DPP4 Assay Kit |
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| 80204 | BPS Bioscience | 96 rxns. | EUR 465 |
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Description: The Fluorogenic DPP4 Assay Kit is_x000D_designed to measure DPP4 activity using purified DPP4 for screening and profiling applications._x000D_ The key to the Fluorogenic DPP4 Assay Kit_x000D_is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter_x000D_plate is required for DPP4 reactions; no time-consuming washing steps are required. The DPP fluorometric substrate is incubated with a_x000D_sample containing DPP4 enzyme to produce a fluorophore that can then be measured_x000D_using a fluorescence reader._x000D_ |
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DPP7 Assay Kit |
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| 80207 | BPS Bioscience | 96 rxns. | EUR 515 |
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Description: The Fluorogenic DPP7 Assay Kit is_x000D_designed to measure DPP7 activity using purified DPP7 for screening and profiling applications._x000D_The key to the Fluorogenic DPP7 Assay Kit is the_x000D_specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is_x000D_required for DPP7 reactions. The fluorometric substrate is incubated with a sample_x000D_containing DPP7 enzyme to produce a fluorophore that can then be measured using a_x000D_fluorescence reader. |
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DPP8 Assay Kit |
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| 80208 | BPS Bioscience | 96 rxns. | EUR 515 |
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Description: The Fluorogenic DPP8_x000D_Assay Kit is designed to measure DPP8 activity using purified DPP8 for screening and_x000D_profiling applications. The key to_x000D_the Fluorogenic DPP8 Assay Kit is the fluorogenic substrate. Using this kit, only one_x000D_simple step on a microtiter plate is required for DPP8 reactions. The fluorometric_x000D_substrate is incubated with a sample containing DPP8 enzyme to produce a fluorophore_x000D_that can then be measured using a fluorescence reader._x000D_ |
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This may occasionally reveal the underlying mechanism by which TAGLN2 would possibly regulate the development of CRC and supply potential therapeutic targets for the remedy of CRC.
