The long-term feeding of a high-concentrate weight loss program (the focus ratio is bigger than 60 %) results in mammary gland inflammatory response in ruminants and decreased high quality in dairy cows and impacts the strong growth of the dairy business.
The primary purpose is intently associated to elevated lipopolysaccharide (LPS) within the physique. On this experiment, a bovine mammary epithelial cell line (MAC-T) was used as a mannequin, and LPS at completely different concentrations (Zero ng/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1000 ng/ml, 10000 ng/ml) was added to the cells.
The cell survival price, oxidative stress indicators, complete lipid droplet space, triglyceride content material and key genes regulating lipid metabolism have been detected by 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide (MTT), assay equipment, microscope commentary and RT-PCR strategies to discover the regulatory mechanism of mammary well being and milk fats synthesis.
The outcomes confirmed that in contrast with these of the management group, the survival charges of cells have been considerably decreased after 9 h of stimulation with 1000 ng/ml and 10000 ng/ml LPS (P<0.01).
The contents of superoxide dismutase (SOD), catalase (CAT) and complete antioxidant capability (T-AOC) in cells have been considerably decreased (P<0.05). In contrast with that of the management group, the content material of malondialdehyde (MDA) in cells was considerably elevated (P<0.05) after stimulation with 10000 ng/ml LPS for 9 h.
After 9 h of stimulation with 100 ng/ml, 1000 ng/ml and 10000 ng/ml LPS, the full lipid drop space and triglyceride (TG) content material of MAC-T cells have been considerably decreased (P<0.05).
The expression ranges of fatty acid synthesis-related genes Acetyl–CoA carboxylase (ACC) and Stearoyl-CoA desaturase 1 (SCD-1) have been considerably decreased after 9 h of stimulation with 100 ng/ml, 1000 ng/ml and 10000 ng/ml LPS (P<0.05), whereas the expression ranges of Fatty Acid synthetase (FAS) have been considerably decreased after stimulation with 1000 ng/ml and 10000 ng/ml LPS (P<0.05).
TG synthesis by the associated gene Diacylglycerol acyltransferase-1 (DGAT1) was considerably decrease than that of the management group after stimulation with 1000 ng/ml and 10000 ng/ml LPS for 9 h (P<0.05), and Diacylglycerol acyltransferase-2 (DGAT2) additionally confirmed a big lower after 10000 ng/ml LPS stimulation (P<0.05).
In conclusion, including completely different concentrations of LPS to MAC-T cells not solely led to a lower in cell exercise, leading to oxidative harm, but in addition affected fatty acid and TG synthesis, which can finally be intently associated to the lower in milk fats synthesis.
Impact of inhibiting ACAT-1 expression on the expansion and metastasis of Lewis lung carcinoma.
Accumulating proof means that acetyl–CoA acetryltransferase 1 (ACAT-1) might mediate tumor growth and metastasis. Nonetheless, the particular operate served by ACAT-1 in lung most cancers is just not nicely understood.
Due to this fact, the current examine initially verified that ACAT-1 was overexpressed in Lewis lung carcinoma (LLC) tissues in contrast with non-LLC mice and that this overexpression promoted the proliferation, invasion and metastasis of those LLC samples.
Western blotting, immunofluorescence microscopy and circulate cytometry allowed the current examine to find out that the ACAT-1 inhibitor avasimibe considerably diminished the expression of ACAT-1 in LLC in contrast with LLC cells that aren’t handled with avasimibe (P<0.05).
A mix of Cell Counting Equipment-Eight and wound therapeutic assays demonstrated that downregulating ACAT-1 expression sufficiently inhibited the proliferation of LLC cells. Avasimibe promoted LLC cell apoptosis as assessed by a Annexin V/propidium iodide double staining assay.
Moreover, avasimibe inhibited tumor development in vivo and improved immune responses, with tissue biopsies from LLC mannequin mice exhibiting larger ranges of ACAT-1 in contrast with in wholesome controls.
Altogether, the outcomes of the current examine reveal that avasimibe might inhibit the development of LLC by downregulating the expression of ACAT-1, which can thus be a possible novel therapeutic goal for lung most cancers therapy.
The impact and mechanism of tamoxifen-induced hepatocyte steatosis in vitro.
The goal of this examine was to find out the impact and mechanism of tamoxifen (TAM)-induced steatosis in vitro. HepG 2 (Human hepatocellular liver carcinoma cell line) cells have been handled with completely different concentrations of TAM for 72 h.
Steatosis of hepatocytes was decided after Oil Crimson O staining and measurement of triglyceride (TG) focus. The expressions of genes within the TG homeostasis pathway, together with sterol regulatory element-binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein α (C/EBPα), fatty acid synthase (FAS), acetyl–CoA carboxylase (ACC), stearoyl-CoA desaturase (SCD), carnitine palmitoyltransferase 1 (CPT1) and microsomal triglyceride switch protein (MTP), have been examined utilizing quantitative real-time PCR and Western blot evaluation.
Cell proliferation was examined utilizing the cell counting equipment-8 (CCK-8) assay.
We discovered that hepatocytes handled with TAM had:
(1) induced hepatocyte steatosis and elevated hepatocyte TG;
(2) upregulation of SREBP-1c, FAS, ACC, SCD and MTP mRNA expressions (300%, 600%, 70%, 130% and 160%, respectively);
(3) corresponding upregulation of protein expression; and
(4) no distinction in HepG 2 cell proliferation.
Our outcomes recommend that TAM can induce hepatocyte steatosis in vitro and that the enhancement of fatty acid synthesis by means of the upregulations of SREBP-1c and its downstream goal genes (FAS, ACC and SCD) will be the key mechanism of TAM-induced hepatocyte steatosis.
Lipid storage droplet protein 5 reduces sodium palmitate‑induced lipotoxicity in human regular liver cells by regulating lipid metabolism‑associated components.
Lipid storage droplet protein 5 (LSDP5) is particularly expressed in tissues with excessive oxidative metabolism resembling liver and coronary heart. The current examine aimed to explored the position of LSDP5 in sodium palmitate‑induced lipotoxicity in LO2 regular human liver cells.
LO2 cells have been handled with varied concentrations of sodium palmitate (25, 50, 75, 100, 125 and 150 µmol/l) for 12, 24 and 48 h, and cell viability was decided by Cell Counting Equipment‑8. Subsequently, LO2 cells have been uncovered to 100 µmol/l sodium palmitate for 48 h to induce lipotoxicity (Mannequin).
Lipotoxicity Mannequin LO2 cells have been additionally transfected with pCMV5‑LSDP5 overexpression vector, and reactive oxygen species (ROS) manufacturing, mitochondrial membrane potential (MMP) and apoptotic charges have been measured. The contents of non‑esterified fatty acid (NEFA), malondialdehyde (MDA) and superoxide dismutase (SOD) have been additionally measured.
The expression ranges of LSDP5, and apoptosis‑, mitochondrial‑, lipid metabolism‑associated components have been detected utilizing reverse transcription‑quantitative polymerase chain response and western blot assays.
The outcomes indicated that sodium palmitate publicity inhibited cell viability and induced lipotoxicity in LO2 cells. LSDP5 overexpression decreased ROS and apoptotic charges, and diminished NEFA and MDA content material.LSDP5 transfection rescued the lack of MMP and elevated SOD content material in lipotoxicity Mannequin LO2 cells.
As well as, LSDP5 upregulated the expression ranges of B‑cell lymphoma‑2, acetyl‑CoA carboxylase1/2 and fatty acid synthase (Fas), whereas the expression ranges of activated‑caspase‑3, Bcl‑2‑related X protein, cytochrome c, cytochrome c oxidase subunits IV, carnitine palmitoyltransferase 1a and peroxisome proliferator‑activated receptors α ranges have been downregulated.
Mouse Acetyl Coenzyme A (acetyl-CoA) ELISA Kit |
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| abx254659-96tests | Abbexa | 96 tests | EUR 904.8 |
Human Acetyl coenzyme A (acetyl-CoA)ELISA Kit |
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| QY-E05300 | Qayee Biotechnology | 96T | EUR 472.8 |
Acetyl CoA synthetase Antibody |
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| 49335-100ul | SAB | 100ul | EUR 399.6 |
Acetyl CoA synthetase Antibody |
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| 49335-50ul | SAB | 50ul | EUR 286.8 |
Acetyl CoA Carboxylase Antibody |
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| 21583-100ul | SAB | 100ul | EUR 302.4 |
Acetyl CoA Carboxylase Antibody |
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| 21583-50ul | SAB | 50ul | EUR 224.4 |
anti-Acetyl CoA synthetase |
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| YF-PA19911 | Abfrontier | 50 ug | EUR 435.6 |
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Description: Mouse polyclonal to Acetyl CoA synthetase |
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Human Acetyl CoA oxidase ELISA kit |
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| E01A0641-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Human Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Acetyl CoA oxidase ELISA kit |
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| E01A0641-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Human Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Acetyl CoA oxidase ELISA kit |
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| E01A0641-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Human Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Acetyl CoA oxidase ELISA kit |
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| E02A0641-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rat Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Acetyl CoA oxidase ELISA kit |
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| E02A0641-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rat Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rat Acetyl CoA oxidase ELISA kit |
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| E02A0641-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Rat Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Phosphorylated acetyl CoA ELISA kit |
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| E01P0588-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Human Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Phosphorylated acetyl CoA ELISA kit |
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| E01P0588-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Human Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human Phosphorylated acetyl CoA ELISA kit |
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| E01P0588-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Human Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Phosphorylated acetyl CoA ELISA kit |
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| E06P0588-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Goat Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Phosphorylated acetyl CoA ELISA kit |
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| E06P0588-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Goat Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Phosphorylated acetyl CoA ELISA kit |
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| E06P0588-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Goat Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Acetyl CoA oxidase ELISA kit |
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| E07A0641-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Porcine Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Acetyl CoA oxidase ELISA kit |
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| E07A0641-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Porcine Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Pig Acetyl CoA oxidase ELISA kit |
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| E07A0641-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Porcine Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Acetyl CoA oxidase ELISA kit |
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| E04A0641-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Rabbit Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Acetyl CoA oxidase ELISA kit |
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| E04A0641-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Rabbit Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Rabbit Acetyl CoA oxidase ELISA kit |
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| E04A0641-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Rabbit Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Acetyl CoA oxidase ELISA kit |
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| E06A0641-192T | BlueGene | 192 tests | EUR 1524 |
|
Description: A competitive ELISA for quantitative measurement of Goat Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Acetyl CoA oxidase ELISA kit |
|||
| E06A0641-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
|
Description: A competitive ELISA for quantitative measurement of Goat Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat Acetyl CoA oxidase ELISA kit |
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| E06A0641-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
|
Description: A competitive ELISA for quantitative measurement of Goat Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Phosphorylated acetyl CoA ELISA kit |
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| E08P0588-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Canine Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Phosphorylated acetyl CoA ELISA kit |
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| E08P0588-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Canine Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Dog Phosphorylated acetyl CoA ELISA kit |
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| E08P0588-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Canine Phosphorylated acetyl CoA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Acetyl CoA oxidase ELISA kit |
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| E09A0641-192T | BlueGene | 192 tests | EUR 1524 |
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Description: A competitive ELISA for quantitative measurement of Monkey Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Acetyl CoA oxidase ELISA kit |
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| E09A0641-48 | BlueGene | 1 plate of 48 wells | EUR 624 |
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Description: A competitive ELISA for quantitative measurement of Monkey Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Monkey Acetyl CoA oxidase ELISA kit |
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| E09A0641-96 | BlueGene | 1 plate of 96 wells | EUR 822 |
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Description: A competitive ELISA for quantitative measurement of Monkey Acetyl CoA oxidase in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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LSDP5 might produce a protecting impact on sodium palmitate‑induced lipotoxicity in LO2 cells by regulating lipid metabolism‑associated components.
